Sulfaquinoxaline(SQX) ELISA Test Kit Eggs, Fish, Shrimp, Meat (Beef, Chicken And Pork), Honey, Milk, Milk Powder,Water
Sulfaquinoxaline(SQX) ELISA Test Kit
CH86135
Product Description
Sulfaquinoxaline ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Sulfaquinoxaline (SQX) in fish, shrimp, honey, kidney, liver, meat (beef, chicken and pork), milk, serum, urine and water.
The unique features of the kit are:
- Rapid extraction method for various samples with high recovery (>80%).
- High sensitivity (0.2 ng/g or ppb) and low detection limit (0.25 ng/g or ppb for shrimp).
- A quick ELISA assay (less than 1.5 hours regardless of number of samples).
- High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The plate wells are coated with capture antibodies directed against anti-SQX antibodies. SQX standards or sample solution, SQX –HRP conjugate and anti-SQX antibodies are added. Free SQX and SQX-HRP Conjugate compete for the SQX antibody binding sites. At the same time, the anti-SQX antibodies are also bound by the immobilized capture antibodies. Any unbound SQX-HRP Conjugate is then removed in a washing step. The TMB Substrate is added to the wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.
Kit Contents, Storage and Shelf Life
Sulfaquinoxaline ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Sulfaquinoxaline Plate | 1x96-well Plate(8wellsx12 strips) | 2-8°C |
|
SQX Standards: Negative control (white cap tube) 0.2 ng/mL (yellow cap tube) 1.5 ng/mL (orange cap tube) 5.0 ng/mL (pink cap tube) 15 ng/mL (purple cap tube) 50 ng/mL (blue cap tube) 1000 ng/mL (spiking, optional, red cap tube) |
1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL 1.0 mL |
2-8°C
2-8°C
|
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