Ciproflo xacin ELISA Test Kit feed, meat,tissue, milk, serum and urine
Ciproflo*xacin ELISA Test Kit
CH86120
Product Description
Ciproflo*xacin ELISA Test Kit provides a competitive enzyme mmunoassay for the quantitative analysis of Ciproflo*xacin in feed, meat,tissue, milk, serum and urine.
The unique features of the kit are:
- Rapid, high recovery (75 - 95%), and cost-effective extraction methods for various samples.
- High sensitivity (0.1 ng/g or ppb) and low detection limit (0.525 ng/g or ppb) for feed samples.
- High reproducibility.
- A quick ELISA assay (less than 1.5 hour regardless of number of samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The ciproflo*xacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ciproflo*xacin-horseradish peroxidase (Ciproflo*xacin-HRP) conjugate. If the ciproflo*xacin residue is present in the sample, it will compete for the ciproflo*xacin antibody, thereby preventing the Ciproflo*xacin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the ciproflo*xacin residue concentration in the sample.
Kit Contents, Storage and Shelf Life
Ciproflo*xacin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Ciproflo*xacin-coated Plate | 96-well plate (8 wells x 12 strips) | 2-8°C |
|
Ciproflo*xacin Standards: Negative control (white cap tube) 0.1 ng/mL (yellow cap tube) 0.3ng/mL (orange cap tube) 0.9ng/mL (pink cap tube) 2.7ng/mL (purple cap tube) 8.1 ng/mL (blue cap tube) 100ng/mL (spiking, optional, red cap tube) |
1.0mL 1.0mL 1.0mL 1.0mL 1.0mL 1.0mL 1.0mL |
2-8°C |
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