Zearalenone ELISA Kit
CH83015
Zearalenone ELISA Kit provides a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals,bran cereals,DDGS,corn gluten meal,corn germ, corn syrup and animal feed.Up to 96 tests or assaying about 42 samples in duplicate( assuming 12 wells for standards).
Zearalenone ELISA Test Kit Cereals,Bran Cereals,Peanut Meal,DDGS,Corn Gluten Meal,Corn Germ, Corn Syrup And Animal Feed
|
The unique features of the kit are:
- LOD:10μg/kg
- LOQ:25μg/kg .
- Quantitative Range:25-1500μg/kg
- 20min
- recovery for certificated reference material:85%~110%
|
| Warnings and Precautions |
- When compared to quantification by LC/MS or GC/MS, it is not uncommon for immunoassays to report higher analyte concentrations. While LC/MS or GC/MS methods typically measure only a single compound, antibodies used in immunoassays not only recognize the target molecule, but also structurally related molecules, including biologically relevant metabolites. So it is the responsibility of the client to understand the limits of both assays system and to interpret their data accordingly.
- Any variation in operating procedure, pipetting technique, washing technique, incubating time or temperature etc, can cause variation in result.
- Do not use the kit beyond the expiration date.
- Do not mix or substitute reagents with those from other lots or sources.
- Water used to prepare all ELISA reagents and buffers must be deionized or distilled water.
- The standards contain zearalenone. Particular care should be taken. Avoid contact of the reagent with the skin(use gloves).
- Decontamination of the glassware and the zearalenone solutions is best carried out using a sodium hypochlorite (bleach) solution (10%v/v) overnight ( adjust solution with HCl to pH 7.0)
|
| Materials Needed But Not Supplied |
|
1.Microtiter plate reader (450 nm)
2.Vortex mixer(optional)
3.Grinder(mill)
4.20-200μL,100-1000μLmicropipettes
|
5.Multi-channel pipette: 50-300 mL (Optional)
8.ethanol ( purity :ACS)
|
