CH98011

       Domoic Acid(ASP) ELISA Test Kit Water Samples As Well As Shellfish Samples

Product Description

         The Domoic Acid ELISA is an immunoassay for the quantitative and sensitive detection of Domoic Acid. Domoic Acid is one of the toxins associated with amnesiac shellfish poisoning (ASP). This test is suitable for the quantitative and/or qualitative detection of Domoic Acid in water samples as well as shellfish samples. For shellfish samples a sample preparation is required.

Procedure   Overview

      The test is a direct competitive ELISA based on the recognition of Domoic Acid by specific antibodies. Domoic Acid, when present in a sample, and a domoic acid-enzyme-conjugate compete for the binding sites of mouse anti-domoic acid antibodies in solution. The domoicacid antibodies are then bound by a second antibody (goat anti-mouse) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Domoic Acidpresent in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Kit Contents, Storage and Shelf Life

          Domoic Acid ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8C *. The shelf life is 12 months when the kit is properly stored.