CH98012

       Okadaic Acid(DSP) ELISA Test Kit Water Samples As Well As Shellfish Samples

Product Description

          The Okadaic Acid ELISA is an immunoassay for the quantitative and sensitive detection of Okadaic Acid. Okadaic Acid is one of the toxins associated with diarrheic shellfish poisoning (DSP). This test is suitable for the quantitative and/or qualitative detection of Okadaic Acid in water samples as well as shellfish samples.

Procedure Overview

              The test is a direct competitive ELISA based on the recognition of Okadaic Acid by specific antibodies. Okadaic Acid, when present in a sample and an okadaic acid-enzyme-conjugate compete for the binding sites of rabbit anti-okadaic acid antibodies in solution. The okadaic acid antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the plate. After a washing step and addition of the substrate solution, a color signal is produced. The intensity of the blue color is inversely proportional to the concentration of Okadaic Acid present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Kit Contents, Storage and Shelf Life

           Okadaic Acid ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.