Product Description

     Metronidazole Strip Test is a qualitative and rapid lateral flow assay designed to detect Metronidazole residues in honey. This test is designed for field use or reference lab settings.The unique features of the kit are:

l Rapid strip test method – 15 minutes

• High affinity antibody method that requires
• l High sensitivity
• l High reproducibility

Procedure Overview

The assay uses a competitive colloidal gold based format. The sample (200 μL) is added to a clear plastic reaction vessel, and used to resuspend the lyophilized reagents to a uniform pink color in the bottom of the microtiter wells. The sample is incubated briefly (3min) at room temperature to allow the Metronidazole binding protein on the gold particles to engage with any Metronidazole antibiotic present in the sample. The test strip is then inserted into the sample well with the arrows pointing downward initiating capillary flow up the strip. Any gold particles that are not complexed with antibiotics present in the sample will bind to the Metronidazole antibiotic imprinted at the Test line (T-line), forming a signal (red line) at that position. If the Metronidazole binding protein on the gold particle has engaged with antibiotic present in the sample, the gold particle will flow past the T-line and reach the Control line (C-line). For visual interpretation of the test results, T-line signal intensity that is stronger than or the same as the signal at the C-line indicates a negative result. The greater the reduction in signal intensity at the T-line, the greater the concentration of Metronidazole antibiotics present in the sample.

Kit Contents, Storage and Shelf Life

The Metronidazole Strip test has the capacity for 40 determinations. The shelf life is 12 months when the kit is properly stored.

Sensitivity (Detection Limit)