5ppb Pesticide Residue Testing Kit Nodularins DM Microcystin Elisa
Carbamate(OP/C) ELISA Test Kit
CH520014
Microcystins/Nodularins-DM ELISA Test Kit In Water Samples
Product Description
OP/Carbamate Test Kit is for the detection of a wide range of organophosphate (including thiophosphate), and carbamate pesticides in water, (drinking water, ground water, surface water and well water).
The unique features of the kit are:
- High sensitivity.
- High reproducibility..
- A quick assay(less than 2 hours regardless of number of samples).
Procedure Overview
The test is a qualitative, colorimetric assay (modification of the Ellman method) for the detection of organophosphates and carbamates, based on a modification of their inhibition of the enzyme Acetyl Cholinesterase (ACh-E). Ach-E hydrolyzes acetylthiocholine (ATC) which reacts with 5,5’-Dithio-bis(2-Nitrobenzoic Acid) [DTNB] to produce a yellow color which is read at 405 nm. If OP or Carbamate pesticides are present in a sample, they will inhibit ACh-E and therefore the color formation will be reduced or absent depending on their concentration. Detection limits of the various OP/C pesticides differ depending on their ability to inhibit the enzyme (refer to Sensitivity table). If it has been established that only a single OP/C is present, the test can be used in conjunction with
appropriate standards for quantitative testing.
Kit Contents, Storage and Shelf Life
Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Polystyrene test tubes (white caps) | 20 |
2-8°C
|
|
Test tube (blue cap)
|
5 mL | |
|
Test tube (green cap)
|
3 mL | |
|
Pesticide (+)(amber vial)
|
1 mL | |
| OXIDIZER(ambervial) | 1 mL | |
| Oxidizer Diluent (orange cap dropper bottle) | 1.8 mL | |
| NEUTRALIZER(red cap dropper bottle) | 2 mL | |
| Ach-E(lyophilized) (green cap dropper bottle) | 1 | |
| SUBSTRATE, ATC(lyophilized) (blue cap dropper bottle) | 1 | |
| CHROMOGEN (DTNB) (yellow cap dropper bottle) | 2 mL | |
| STOPPING SOLUTION(purple cap dropper bottle) | 2 mL | |
| 100 ul Exact volume pipettes | 22 each | |
| 3 ml Transfer pipets | 2 each | |
| Work station box (WSB) | 1 |
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