High Sensitivity, StrongStability,Nitrofurazone(SEM) ELISA Test Kit

Product Description

       Nitrofurazone (SEM)) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Nitrofurazone in fish, shrimp ,eggs, honey and feed.

The unique features of the kit are:

• high recovery (75%-115%), and cost-effective extraction methods for various samples.
• High sensitivity (0.025 ng/g or ppb) and low detection limit (0.05 ng/g or ppb) for various samples.
• High reproducibility.
• A quick ELISA assay (less than 1 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The SEM-BSA has been coated in the plate wells. During the analysis, sample and the SEM antibody are added along with secondary antibody, tagged with a peroxidase enzyme. If the SEM residue is present in the sample, it will compete for the SEM antibody, thereby preventing the antibody from binding to the SEM-BSA attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the SEM residue concentration in the sample.

Sensitivity (Detection Limit)