High Sensitivity 30min Pesticide Residue Testing Kit 0.075ppb Glyphosate Testing Kit
Glyphosate ELISA Test Kit
CH520365
Simple Operation, High Sensitivity, Strong Stability Glyphosate ELISA Test Kit
Product Description
The Glyphosate ELISA Test Kit is an immunoassay for the quantitative and sensitive screening of Glyphosate in water samples. This test is suitable for the quantitative and/or qualitative screening of Glyphosate in groundwater, surface water, well, and tap water samples.Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.
Procedure Overview
The test is a direct competitive ELISA based on the recognition of Glyphosate by polyclonal antibodies. The sample to be tested is derivatized (please refer to Section D, Test Preparation) and then added to microtiter wells coated with goat anti-rabbit antibodies. A rabbit anti-Glyphosate antibody solution is added to the wells with the derivatized samples and allowed to incubate for 30 minutes. The Glyphosate enzyme conjugate is then added and a competitive reaction occurs between the Glyphosate, which may be present in the sample, and the enzyme labeled Glyphosate for the binding sites of the rabbit anti-Glyphosate antibodies bound by the goat anti-rabbit antibodies immobilized on the microtiter plate. The reaction is allowed to continue for 60 minutes.After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Glyphosate present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
Kit Contents, Storage and Shelf Life
Glyphosate ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.
| Kit Contents | Amount | Storage |
| Glyphosate Plate | 1 x 96-well Plate (8 wells x 12 strips) | 2-8°C |
|
Glyphosate Standards: Negative control 0.075ng/mL 0.2ng/mL 0.5ng/mL 1.0ng/mL 4.0ng/mL Control at 0.75 ± 0.2 ppb |
2.0 mL 2.0 mL 2.0 mL 2.0 mL 2.0 mL 2.0 mL 2.0 mL |
2-8°C
|
| Glyphosate Antibody #1 | 6 ml | 2-8°C |
| HRP-Conjugated Antibody #2 | 6 ml | |
| 5X Wash Solution | 100 mL | |
| Sample Dilution Buffer | 30 mL | |
| Stop Buffer | 12 mL | |
| TMB Substrate | 16 mL | |
| Assay Buffer | 125mL | |
| Derivatization Reagent | 3X100µL | |
| Derivatization Reagent Diluent | 3X4 mL |
* If you are not planning to use the kit for over 3 months, store Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.
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