Aflatoxins B1 Fluorescence Quantitative Rapid Detection Kit

                                                                       CH60072

AFB₁ fluorescence quantitative detection reagent applies the principle of competitive suppression immunochromatography. AFB₁ in the sample binds to the specific monoclonal antibody labeled with fluorescent microspheres during the flow process, and then inhibits the binding of the antibody to AFB₁- BSA conjugate on the NC membrane detection line. The fluorescence signal is generated after excitation at a specific wavelength, and the intensity of the signal is inversely proportional to the AFB₁ content in the sample.

Scope of Application

This product is suitable for the quantitative detection of AFB₁ in feedstuff and some finished feed.

Packaging Strength

48 T/box

Product Composition

• Test card: 48 copies

• Microsphere lyophilized product: 48 wells

• Sample diluent: 1 vial

• Data card: 1

• Instructions for Use: 1

Required Reagents and Instrument/Consumables

• Portable fluorescence analyzer

• Thermostatic incubator

• Pipette (20-200µL,100-1000µL)

• Consumables: Pipette tip, disposable gloves, centrifuge tube

• Sample extract: 50% absolute ethanol-pure water solution (volume ratio)

Performance Indicators

Curve range: 1-50 µg/kg

Sample Pretreatment

• Weigh 5.00±0.02 g of homogenized (20-mesh sieve) sample into a 50 mL centrifuge tube.
• Add 25 mL of 30% ethanol extract, cover the vial tightly, vortex with a vortex mixer for 3 min, and centrifuge at 4,000 rpm for 2 min at room temperature; collect 100 µL of supernatant into a new centrifuge tube, add 500 µL of sample diluent, and mix well for later use.

Peanut meal

• Weigh 5.00±0.02 g of homogenized (20-mesh sieve) sample into a 50 mL centrifuge tube.
• Add 25 mL of 30% acetonitrile extract, cover the vial tightly, vortex with a vortex mixer for 3 min, and centrifuge at 4,000 rpm for 2 min at room temperature; collect 100 µL of supernatant into a new centrifuge tube, add 500 µL of sample diluent, and mix well for later use.