Heat-stable Alpha-Amylase HT-800

Low pH Tolerate Food-Grade Liquefaction Enzyme For Starch Sugar

  Robust Activity ,

Superior Thermo-stability ,

Adapted to Low pH Conditions

DESCRIPTION

• HT-800 is a robust Heat-stable Alpha-Amylase (EC 3.2.1.1, 1,4-α-D-glucan glucanohydrolase) produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. As an endoamylase, HT-800 can quickly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
• HT-800 is a robust Heat-stable Alpha-Amylase adapted to low pH conditions for the production of starch sugar.

PRODUCT CHARACTERISTICS

EFFECT OF PH & TEMPERATURE ON ACTIVITY:

• HT-800 can work at pH ranging from 5.0∼7.0 with its optimal pH being 5.4∼6.0.
• The exact pH optimum depends on the actual processing conditions, including temperature, substrate concentration and reaction time.
• For interval liquefaction, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95∼ 97°C and maintains highly active at 100°C.
• For consecutive liquefaction, HT-800 demonstrates exceptional thermal stability at temperature range from 105 to 110°C and keeps on liquefying efficiently for 5 to 7 minutes.

CONCENTRATION OF CALCIUM

• The presence of calcium increases the thermal stability of HT-800.
• This enzyme requires a very low calcium concentration of 20 to 50 ppm (dry solids basis) to ensure its enzyme stability.

INHIBITORS

• Copper, titanium, and cobalt ions are moderate inhibitors for HT-800.
• Aluminum, lead and zinc ions act as strong inhibitors.

USAGE GUIDELINES

• For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry, adjust pH 5.4 to 6.0, then add this product at a dosage rate of 0.10 to 0.35 kg/TDS.
• Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.
• In case the starch content is above 35%, recommended dosage rate is from 0.35 to 0.4kg/TDS.
• Exact dosage rate depends on the actual situation such as starch substrate, liquefaction equipment, processing conditions, etc.
• To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.

PACKAGING, STORAGE AND SHELF-LIFE: