Heat-stable Alpha-Amylase HA-470

High Concentration Alpha-Amylase for Ethanol Porduction

Halal and Kosher Certified

DESCRIPTION

HA-470 is a high concentration Heat-stable Alpha-Amylase produced from strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. HA-470 is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

As a very robust Alpha-Amylase with superior thermal and pH stability, HA-470 enable highly efficient liquefaction in production of ethanol.

PRODUCT CHARACTERISTICS

EFFECT OF PH

HA-470 is active at pH from 5.2 to 6.5. For maximum activity, the optimal pH for HA-470 is 5.4 to 6.0 ( See Figure 1). The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and reaction time.

EFFECT OF TEMPERATURE

For interval liquefaction, the temperature range of HA-470 is 80 to 100°C, the optimum temperature range is 83 to 86°C. It liquefies starch substrates promptly at 90 to 95ºC and maintains highly active at 100ºC. For consecutive liquefaction, HA-470 demonstrates exceptional thermo-stability at a temperature range of 105 to 110ºC, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently ( See Figure 2).

INHIBITORS

Copper, titanium, and cobalt ions are moderate inhibitors for HA-470. Aluminum, lead and zinc ions act as strong inhibitors.

USAGE GUIDELINES

In general, first adjust the pH to 5.4 to 6.0, then add HA-470. Calcium is not needed. After this enzyme is added, heat the slurry to liquefy at a temperature range of 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12 ( See Figure 3 and 4).

Exact dosage rate of HA-470 depends on the kind of starch, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point of dosage rate of HA-470 is 0.07 to 0.2kg/TDS.

PACKAGING

HA-470 is packaged in 25kg, 30kg HDPE drums or 1125kg IBC totes. Customized packaging is available upon request.

STORAGE

HA-470 has been formulated for its optimal stability. Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be eighteen months provided that this product is stored properly at 0∼10°C. Prolonged storage and/or adverse conditions, such as higher temperatures, may lead to higher dosage requirement, thus should be avoided.

SAFETY & ENZYME HANDLING

Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet of this product.