High Concentration Liquefaction Enzyme HT-800
High Concentration Liquefaction Enzyme Heat - Stable Alpha Amylase HT-800 for Food Use
Heat-stable Alpha-Amylase HT-800
Food Grade Heat-stable Alpha-Amylase Low pH Tolerate For Starch Sugar
Halal & Kosher Certified
DESCRIPTION
- HT-800 is a low pH tolerated Heat-stable Alpha-Amylase ( EC 3.2.1.1 1,4-α-D-glucan glucanohydrolase) produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes.
- As an endoamylase, HT-800 can quickly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
HT-800 is a robust Heat-stable Alpha-Amylase tolerates to low pH conditions specially designed for the production of starch sugar.
PRODUCT CHARACTERISTICS
| Declared Enzyme | Alpha-Amylase |
| Systematic Name | 1,4-α-D-glucan glucanohydrolase |
| EC Number | EC 3.2.1.1 |
| Activity | 80,000 U/ml (Minimum) |
| Appearance | Sepia brown |
| Physical Form | Liquid |
| Product pH | 5.0 to 7.0 |
| Specific gravity | ≤1.25 g/ml |
EFFECT OF PH AND TEMPERATURE ON ACTIVITY
| PH | TEMPERATURE |
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HT-800 is can work at pH from 5.0 to 7.0.
For maximum activity, the optimal pH range for HT-800 is 5.4 to 6.0.
The exact pH optimum depends on the actual processing conditions, including temperature, substrate concentration and reaction time, etc.
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For interval liquefaction, the optimum temperature for HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C.
For consecutive liquefaction, HT-800 demonstrates an exceptional thermal stability from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.
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CONCENTRATION OF CALCIUM
- The presence of calcium increases the thermal stability of HT-800.
- This enzyme requires a very low calcium concentration of 20 to 50 ppm (dry solids basis) to ensure its enzyme stability.
INHIBITORS
- Copper, titanium, and cobalt ions are moderate inhibitors for HT-800.
- Aluminum, lead and zinc ions act as strong inhibitors for HT-800..
USAGE GUIDELINES
- For industrial application of one-time spray liquefaction, first prepare 30∼35% w/w starch slurry, adjust pH 5.4∼6.0, then add this product at a dosage rate of 0.20∼0.30 kg/TDS.
- Liquefy at 105∼107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value.
- In case the starch content is above 35%, recommended dosage rate is 0.30∼0.4kg/TDS.
- Exact dosage of HT-800 depends on the actual processing conditions such as starch substrate, liquefaction equipment, pH, temperature, processing time, etc.
- To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
PACKAGING, STORAGE & SHELF-LIFE
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