Heat-stable Alpha-Amylase HS-320

Food Grade NON-GMO High Temperature Liquefaction Amylase 

Reduce viscosity quickly, 

Eliminate non-fermentable sugars,

Decrease  formation of chromophore,

Robust Alpha-Amylase activity capable to liquefy

Starch materials of tapioca/cassava, corn, wheat,rice, etc.

PRODUCT DESCRIPTION:

• HS-320 is Heat stable Alpha-Amylase produced from  NON-GMO strains of Bacillus licheniformis through submerged fermentation and refining extraction process. 
• HS-320 is an endoamylase, which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

USAGE GUIDELINES:

• For industrial application of one-time spray liquefaction, first prepare 30∼35% w/w starch slurry, adjust pH 6.0 ∼ 6.2, add calcium ion 50∼70 mg/L, then add HS-320 at a dosage rate of 0.4 ∼0.6 kg/TDS. Liquefy at 105∼110°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at a temperature above 95°C for 90∼ 120 minutes so as to reach required DE value. If calcium ion concentration in tap water is already sufficient, it is no need to add calcium.
• The actual dosage rate of HS-320 depends on the actual situation including application fields, starches, substrate concentration, temperatures, pH and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to the routine use of HS-320.

EFFECT OF TEMPERATURE:

• Optimum temperature of HS-320 for interval liquefaction is above 90°C.
• HS-320 liquefies starch substrates efficiently at 95 to 97°C and is still highly active at 100°C.
• This product demonstrates exceptional thermal stability at temperatures from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.
• At a temperature above 105°C, the enzyme stability decreases rapidly.
• The exact temperature optimum depends on actual processing conditions including pH, substrate concentration and processing time.

EFFECT OF PH:

• HS-320 is active over a wide pH range, depending on the application.
• For maximum activity, the optimal pH is 6.0 to 6.4.
• The stable pH range is 5.0 to 10.0 at 25°C.
• The exact pH optimum depends on actual process conditions, including temperature, substrate concentration and processing time, etc.

INHIBITORS:

• Copper, titanium, and cobalt ions are moderate inhibitors for HS-320
• Aluminum, lead and zinc ions act as strong inhibitors

PACKAGING, SHELF-LIFE & STORAGE: