Heat-stable Alpha-Amylase HA-400FG for Alcohol & Brewing

  Low pH tolerate, 

Quickly decreases starch viscosity,  

Save processing time

 Reduce cost

DESCRIPTION

• Alpha-Amylase HA-400 is derived from Bacillus licheniformis using submerged fermentation and refining extraction techniques. HA-400 is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
• As a very robust Alpha-Amylase with superior thermal and pH stability, HA-400 is applied to brewing industry such as beer and alcohol.

PRODUCT CHARACTERISTICS

• Declared Enzyme: Alpha-Amylase
• Systematic Name: EC 3.2.1.1,1,4-α-D-glucan glucanohydrolase
• Activity: 40,000 U/ml (minimum)
• Appearance: Light to dark brown liquid
• Product pH: 5.0 to 7.0
• Specific gravity: 1.10-1.25 g/ml

EFFECT OF PH

• The effective pH range for HA-400FG is from 4.8 to 6.0 with its optimal pH range from 5.0 to 5.9. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.

EFFECT OF TEMPERATURE

• For interval liquefaction, HA-400FG works at temperatures from 80 to 100°C with it optimum temperature range from 83 to 86°C. It can liquefy starch substrates promptly at 90 to 95ºC and maintain highly active at 100ºC.
  For consecutive liquefaction, HA-400FG demonstrates its exceptional thermo-stability at a temperature range of 105 to 110ºC and keeps on liquefying efficiently for 5 to 7 minutes.

CONCENTRATION OF CALCIUM

• The presence of calcium increases the thermal stability of HA-400. This enzyme requires a very low calcium concentration and calcium additions of 20 to 50 ppm (dry solids basis) are sufficient to ensure enzyme stability.

INHIBITORS

• Copper, titanium, and cobalt ions are moderate inhibitors for HA-400. Aluminum, lead and zinc ions act as strong inhibitors.

USAGE GUIDELINES

In general, first adjust the pH to 5.0 to 5.9, then add HA-400FG. After this enzyme is added, heat the slurry to liquefy at a temperature range of 83 to 86ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12.

Dosage of HA-400FG is dependent on the actual processing conditions such as kinds of starch, substrate concentration, process temperature, pH and reaction time, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.4 to 0.8 kg/TDS.