Acid Protease AP-520

 Cost-effective, Promote yeast growth, Enhance fermentation, Enable higher alcohol yields

DESCRIPTION

• Acid Protease AP-520 is a food grade acid protease enzyme produced from Aspergillus niger using submerged fermentation, extraction, and refining techniques. AP-520 can effectively hydrolyze proteins under acidic conditions.
• AP-520 can be used in the production of potable alcohol, food and beverage where protein hydrolysis is needed under low pH conditions.

PRODUCT CHARACTERISTICS

EFFECT OF pH

• The effective pH range for the activity of AP-520 is approximately 2.0 to 6.0, with an optimum performance at pH 2.5 to 4.0.

EFFECT OF TEMPERATURE

• The activity of AP-520 is effective in the range from 30°C to 65°C, with an optimum performance obtained at 30°C to 50°C.
• The exact temperature optimum will be influenced by several process variables including time, pH, and substrate nature andsubstrate concentration.

EFFECT OF METAL IONS

• AP-520 is activated by Mn2+, Ca2+ and Mg2+ ions. Heavy metal ions such as Cu2+, Hg2+ and Al3+ will inhibitthe enzyme activity of AP-520.

APPLICATION GUIDELINES

• In intermittent fermentations, the entire dose of AP-520 should be added all at once after the fermentation tank is filled approximately 30%. In continuous fermentations,
• AP-520 should be added continually, with the dosage rate dependent on the amount of mash fed at the inlet of the fermentation tank.

DOSAGE RECOMMENDATION

• The typical dosage rate for AP-520 is 0.01% to 0.02% w/w (dry solids basis) and this dosage level is recommended as a starting point for optimizing the enzyme dosage.
• The optimum enzyme dosage will be dependent on both the raw materials used (substrate nature and concentration) and the processing conditions.