Glucoamylase PUREMEI PG

NON-GMO Saccharification Enzyme for Ethanol

DESCRIPTION

Glucoamylase PUREMEI PG is made from NON-GMO strains of Aspergillus niger through submerged fermentation, extraction and refining processes. PUREMEI PG has a high conversion rate from starchy substrates into fermentable sugars. It can hydrolyze α-D-1, 4 glucosidic bonds from the non-reducing end of starch one after another. It can also hydrolyze the α-D-1, 6 glucoside branch bonds of starch and cleave the α-1, 3 bonds to release glucose.

PUREMEI PG is designed with a robust glucoamylase activity for saccharification of liquefied starch mash in productionfuel ethanol . Use of PUREMEI PG provides higher ethanol yields, faster fermentation and less starch residues.

PRODUCT CHARACTERISTICS

APPLICATION CONDITIONS

The optimal pH for PUREMEI PG ranges from 4.0 to 4.5. See Figure 1.

FIGURE 1. EFFECT OF PH ON ACTIVITY

Substrate: 4%(w/v) Soluble Starch

Temperature: 60℃

Time: 60 min

The effective temperature for PUREMEI PG is from 30 to 65°C, and its optimal temperature range is from 55 to 60°C. See Figure 2.

FIGURE 2. EFFECT OF TEMP ON ACTIVITY

Substrate: 4%(w/v) Soluble Starch

pH: 4.2

Time: 60 min

ADVANTAGES AND BENEFITS

The robust glucoamylase activity of PUREMEI PG is capable to saccharify liquified mash from various sources including:

• corn
• milo
• barley
• wheat
• rice
• potatoes 
• tapioca

For ground-grain processing plants using simultaneous saccharification fermentation (SSF), PUREMEI PG may be added directly to the fermenter.

For ground-grain dry mill plants and for wet mill plants using a separate pre-saccharification step prior to fermentation, PUREMEI PG may be added to the cooling mash or liquefied starch when the temperature reaches 55 to 60°C. Temperatures above 65°C can result in loss of activity rapidly.

DOSAGE GUIDELINES

PUREMEI PG is generally added at a level of 0.065 to 0.085% w/w (dry solids basis).

The optimal dose depends on the actual processing conditions including raw materials, temperature, reaction time, pH and the desired results in individual ethanol plants.

PACKAGING, SHELF-LIFE AND STORAGE

PUREMEI PG is packaged in 25kg, 30kg HDPE plastic drums or 1125 kg IBC totes. Customized packaging is also available upon request.

Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight.

Its shelf-life will be eighteen months if this product is stored properly at 0～10°C.

Prolonged storage and/or adverse conditions such as higher temperatures may lead to higher dosage requirement, thus should be avoided.

SAFE HANDLING OF ENZYME 

Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.